Analysis effect of shade level on the physiological and anatomical characteristics of hybrid Phalaenopsis orchid at the acclimatization stage

Production of hybrid Phalaenopsis seedlings is generally applied by in vitro culture techniques. The final stage acclimatization. acclimatization crucial because the plantlets must adapt to ex-vitro environment. Shade supports plantlet growth during early stages This study aims determine effect shade level on physiological and anatomical characteristics orchid at stage. research used shading level. Plantlets were shaded 40%, 55%, 70%. Parameters measured included: photosynthetic pigment content, number area leaves, length roots, stomata density, size fresh weight. a completely randomized design with one factor ten replications. Data analyzed using ANOVA test LSD significance 5% (p<0.05). results showed highest content 55% shade, there was difference stomatal guard cells between ex leaves 40% shade. did not affect leaf growth, weight density. However, it affected orchids. most optimal Keywords : , Abstrak Produksi bibit hibrid umumnya dilakukan dengan teknik kultur . Tahap akhir dari adalah aklimatisasi. Aklimatisasi merupakan tahap paling penting karena planlet harus beradaptasi di lingkungan Naungan menunjang pertumbuhan selama awal Penelitian ini bertujuan untuk mengetahui pengaruh tingkat naungan terhadap karakteristik fisiologis dan anatomis anggrek pada menggunakan naungan. Planlet diberi paranet Parameter yang diukur meliputi: kandungan pigmen fotosintesis, jumlah luas daun, panjang akar, densitas stomata, ukuran berat segar. rancangan acak lengkap satu faktor 10 ulangan. dianalisis Uji taraf signifikasi (p< 0,05). Hasil penelitian menunjukkan bahwa fotosintesis tertinggi terdapat danterdapat perbedaan sel penutup antara daun 40%. Tingkat tidak mempengaruhi segar serta namun hibrid. Pertumbuhan berada 55%. Kata Kunci aklimatisasi,